Abstract
Autism Spectrum Disorder (ASD) is a neurodevelopmental condition characterized by challenges in social communication, repetitive behaviors, and restricted interests. Emerging research has emphasized the significance of metabolic dysfunctions in the pathophysiology of ASD, including abnormalities in energy production, mitochondrial dysfunction, and oxidative stress. This study explores metabolic alterations specific to brain tissues, cell types and blood in individuals with ASD by integrating omics data with the human metabolic network. We identified significant brain-specific metabolic changes in ASD, underscoring hypometabolism and reduced glucose utilization. Additionally, alterations in pathways related to fatty acid metabolism, amino acid metabolism, and vitamin metabolism were observed. The analysis of cell-specific metabolic activities in the ASD brain showed alterations in pathways in astrocytes, microglia, and oligodendrocytes. Metabolic gene expression in peripheral blood mononuclear cells (PBMC) samples also showed potential as diagnostic biomarkers. These findings offer insights into metabolic interventions in ASD, as well as potential biomarkers and therapeutic targets for ASD.