Abstract
Stress has a significant mental health problem of the 21st century. The number of people suffering from stress is increasing rapidly. Thus, easy-to-use, inexpensive, and accurate biomarkers are needed to detect stress during its inception. Early detection of stress-related diseases allows people to access health-care services and leads to the development of new therapies. Thus, for the early detection of stress, a biomarker would be beneficial. We aim to find if there are significant differences between stressed and non-stressed groups of participants and which brain region gets impacted during stress. We conducted experiments to acquire EEG signals to identify the most significant brain region that gets affected due to stress. This research investigates if Higuchi Fractal Dimension (HFD) extracted from EEG signals can act as potential stress biomarkers. A gamified mobile application, known as Color Word and Memory Test (CWMT), was developed, inspired by the well-known Stroop Test, to elicit mental stress at two different difficulty levels. A MUSE headband with four EEG sensors (TP9, AF7, AF8, TP10) was used to collect the EEG signals at the two difficulty levels. The Higuchi Fractal Dimension (HFD) was extracted from EEG signals acquired from 32 participants while they were exposed to stress stimuli (using the proposed CWMT application) and we then performed three experimental analyses, i.e., Analysis I, II and III. Analysis I was performed on all the 32 participants performing low and high difficulty tasks on CWMT application. Analysis II was performed on sub-groups of participants. These sub-groups were made based on score obtained by participants while using CWMT application. Analysis II aimed to find the most significantly impacted brain region during stress. For Analysis III, we used the same sub-groups as Analysis II and it aimed to identify any differences between the left and right hemispheres during stress. We conducted the statistical analysis, and p-values were calculated between the two groups (non-stressed and stressed) to detect EEG channels and brain frequency significantly associated with stress. We performed three experimental analyses (Analysis I and II are intra-hemisphere, and analysis III is inter-hemisphere). In Analysis I, we inferred that beta and alpha frequencies from the AF8 region of the brain are affected during stress. In Analysis II, we inferred that beta waves from the AF8 region are a characteristic indicator of stress. In Analysis III, we identified significant differences between the left and right parts of the brain during stress. We found a significant difference (p<0.05) between HFD value in stress and non-stressed groups in the AF8 region. Our results indicated that stressed patients have a significantly higher value of HFD in the frontal areas.